期刊
ENDOCRINOLOGY
卷 157, 期 8, 页码 3023-3035出版社
ENDOCRINE SOC
DOI: 10.1210/en.2016-1035
关键词
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资金
- National Natural Science Foundation of China [81370859]
- 111 Project [B08011]
- Austrian Science Fund (FWF) [V 216] Funding Source: researchfish
- Austrian Science Fund (FWF) [V216] Funding Source: Austrian Science Fund (FWF)
Cancer-associated fibroblasts (CAFs) play a vital role in malignant transformation and progression of prostate cancer (PCa), and accumulating evidence suggests an enhancing effect of estrogens on PCa. The present study aimed to investigate the possible origin of prostate CAFs and the effects of estrogen receptors, G protein-coupled receptor 30 (GPR30) and estrogen receptor (ER)-alpha, on stromal cell activation. High expression of fibroblast activation protein (FAP), CD44, and nonmuscle myosin heavy chain B (SMemb) accompanied by low expression of smooth muscle differentiation markers was found in the stromal cells of PCa tissues and in cultured human prostate CAFs. Additionally, SMemb expression, which is coupled to cell phenotype switching and proliferation, was coexpressed with FAP, a marker of activated stromal cells, and with the stem cell marker CD44 in the stromal cells of PCa tissue. Prostate CAFs showed high GPR30 and low ER alpha expression. Moreover, GPR30 was coexpressed with FAP, CD44, and SMemb. Furthermore, the study demonstrated that the overexpression of GPR30 or the knockdown of ER alpha in prostate stromal cells induced the up-regulation of FAP, CD44, Smemb, and the down-regulation of smooth muscle markers. The conditioned medium from these cells promoted the proliferation and migration of LNCaP and PC3 PCa cells. GPR30 knockdown or ER alpha overexpression showed opposite effects. Finally, we present a novel mechanism whereby GPR30 limits ER alpha expression via inhibition of the cAMP/protein kinase A signaling pathway. These results suggest that stem-like cells within the stroma are a possible source of prostate CAFs and that the negative regulation of ER alpha expression by GPR30 is centrally involved in prostate stromal cell activation.
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