Magnetite nanoparticles coated with citric acid are not phytotoxic and stimulate soybean and alfalfa growth

In this work, the internalization and distribution of citric acid-coated magnetite nanoparticles (here, Fe3O4-NPs) in soybean and alfalfa tissues and their effects on plant growth were studied. Both legumes were germinated in pots containing an inert growing matrix (vermiculite) to which Hoagland solution without (control, C), with Fe3O4-NPs (50 and 100 mg iron L-1, NP50 and NP100), or with the same amount of soluble iron supplied as FeEDTA (Fe50, Fe100) was added once before sowing. Then, plants were watered with the standard nutrient solution. The observation of superparamagnetic signals in root tissues at harvest (26 days after emergence) indicated Fe3O4-NPs uptake by both legumes. A weak superparamagnetic signal was also present in the stems and leaves of alfalfa plants. These findings suggest that Fe3O4-NPs are readily absorbed but not translocated (soybean) or scarcely translocated (alfalfa) from the roots to the shoots. The addition of both iron sources resulted in increased root weight; however, only the addition of Fe3O4-NPs resulted in significantly higher root surface; shoot weight also increased significantly. As a general trend, chlorophyll content enhanced in plants grown in vermiculite supplemented with extra iron at pre-sowing; the greatest increase was observed with NP50. The only antioxidant enzyme significantly affected by our treatments was catalase, whose activity increased in the roots and shoots of both species exposed to Fe3O4-NPs. However, no symptoms of oxidative stress, such as increased lipid peroxidation or reactive oxygen species accumulation, were evidenced in any of these legumes. Besides, no evidence of cell membrane damage or cell death was found. Our results suggest that citric acid-coated Fe3O4-NPs are not toxic to soybean and alfalfa; instead, they behave as plant growth stimulators.

Automated summary

The study indicated that Fe3O4-NPs were taken up by soybean and alfalfa but scarcely translocated to shoots. Addition of Fe3O4-NPs resulted in increased root weight, root surface, and shoot weight, as well as enhanced chlorophyll content. Fe3O4-NPs affected the activity of antioxidant enzyme catalase in roots and shoots, but did not cause oxidative stress or cell damage in the legumes. These nanoparticles behaved as plant growth stimulators rather than exhibiting toxicity to soybean and alfalfa.