Highly selective recognition of intramolecular parallel G-quadruplex using a chiral supramolecular probe

Owing to their chiral and self-assembly features, chiral supramolecules exhibit superior recognition and catalytic abilities. Here, we construct a chiral H-aggregates based on a cyanine dye, 3,3'-di(3-sulfopropyl)-4,5,4'5'-dibenzo-9-methyl-thiacarbocyanine triethylammonium salt (MeCy), by spontaneous symmetry breaking in the presence of NaNO3. The chiral H-aggregates could specially recognize parallel DNA G-quadruplexes among other DNA types, probably by a three-step process, which comprises the amplified chiral induction, the disassembly of chiral H-aggregates and the binding of the MeCy monomer. The MeCy monomer binding to DNA parallel G-quadruplex led to a lighting-up fluorescence intensity obviously. Chiral supramolecules targeted to DNA G-quadruplex was proposed, and its transformation process was revealed, and thus potentially provided an excellent probe for DNA parallel G-quadruplexes.

Automated summary

Chiral supramolecules exhibit superior recognition and catalytic abilities due to their chiral and self-assembly features. The constructed chiral H-aggregates in this study can specifically recognize DNA G-quadruplexes and enhance fluorescence intensity through binding of MeCy monomer.