期刊
DEVELOPMENTAL CELL
卷 56, 期 3, 页码 325-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2020.12.003
关键词
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资金
- NSF Graduate research fellowship [DGE-1553798]
- NIH [R01DK064380]
- Crnic INCLUDE
Primary cilia are sensory organelles that use membrane and cytoplasm compartmentalization to signal events. The study found that RAB19 plays a key role in coordinating cortical clearing and ciliary membrane growth, essential for ciliogenesis. Inhibiting actomyosin contractility can substitute for the function of the RAB19 complex and restore ciliogenesis in knockout cells.
Primary cilia are sensory organelles that utilize the compartmentalization of membrane and cytoplasm to communicate signaling events, and yet, how the formation of a cilium is coordinated with reorganization of the cortical membrane and cytoskeleton is unclear. Using polarized epithelia, we find that cortical actin clearing and apical membrane partitioning occur where the centrosome resides at the cell surface prior to ciliation. RAB19, a previously uncharacterized RAB, associates with the RAB-GAP TBC1D4 and the HOPS-tethering complex to coordinate cortical clearing and ciliary membrane growth, which is essential for ciliogenesis. This RAB19-directed pathway is not exclusive to polarized epithelia, as RAB19 loss in non-polarized cell types blocks ciliogenesis with a docked ciliary vesicle. Remarkably, inhibiting actomyosin contractility can substitute for the function of the RAB19 complex and restore ciliogenesis in knockout cells. Together, this work provides a mechanistic understanding behind a cytoskeletal clearing and membrane partitioning step required for ciliogenesis.
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