AAA plus ATPases: structural insertions under the magnifying glass

AAA+ ATPases are a diverse protein superfamily which power a vast number of cellular processes, from protein degradation to genome replication and ribosome biogenesis. The latest advances in cryo-EM have resulted in a spectacular increase in the number and quality of AAA+ ATPase structures. This abundance of new information enables closer examination of different types of structural insertions into the conserved core, revealing discrepancies in the current classification of AAA+ modules into clades. Additionally, combined with biochemical data, it has allowed rapid progress in our understanding of structure-functional relationships and provided arguments both in favour and against the existence of a unifying molecular mechanism for the ATPase activity and action on substrates, stimulating further intensive research.

Automated summary

AAA+ ATPases are a diverse protein superfamily that drive various cellular processes. Advances in cryo-EM have provided more detailed insights into their structures, leading to a better understanding of their functional relationships and revealing discrepancies in their classification.