期刊
CRYOBIOLOGY
卷 98, 期 -, 页码 194-200出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2020.11.001
关键词
Cryoprotectant; Ram; Slow freezing; Spermatozoa
资金
- CNPq (National Council for Scientific and Technological Development) [434302/2018-0]
- FAPERJ (Carlos Chagas Filho Foundation for Support to Research in the State of Rio de Janeiro)
- CAPES [001]
The addition of antifreeze proteins (AFP), especially type I, in ram sperm cryopreservation can increase sperm protection, enhance plasma membrane integrity, and improve the percentage of normal sperm.
Antifreeze proteins (AFP) play an important role in cellular survival at sub-zero temperatures. This study assessed the effect of AFP type I or III in semen extender (TRIS-egg yolk) for ram sperm cryopreservation. Pooled semen of four rams were allocated into five treatments: Control (CONT, without AFP); AFP Type I [0.1 (AFPI-0.1) or 0.5 (AFPI-0.5) ug/mL]; or III [0.1 (AFPIII-0.1) or 0.5 (AFPIII-0.5) mu g/mL], and then frozen in six replicates. Treatments affected kinetic parameters, plasma membrane integrity and morphology (P < 0.05). The AFPIII-0.1 presented lesser total motility. Linearity was greater in AFPI-0.1, AFPI-0.5 and AFPIII-0.5 and straightness was greater in all AFP-supplemented extenders. Plasma membrane integrity was greater in AFPI-0.1 and AFPI-0.5. All AFP groups had greater percentage of normal sperm than CONT. No differences (P > 0.05) were observed in hypoosmotic test, sperm acrosome status, mitochondrial activity, chromatin condensation, perivitelline membrane binding rate and lipoperoxidation. In conclusion, the use of AFP, predominantly type I, may increase sperm cell protection during cryopreservation, with no adverse effect on potential fertilization capacity or increase in reactive oxygen species, being a potential cryoprotectant to ram sperm.
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