Breathomics: Review of Sample Collection and Analysis, Data Modeling and Clinical Applications

Metabolomics research is rapidly gaining momentum in disease diagnosis, on top of other Omics technologies. Breathomics, as a branch of metabolomics is developing in various frontiers, for early and noninvasive monitoring of disease. This review starts with a brief introduction to metabolomics and breathomics. A number of important technical issues in exhaled breath collection and factors affecting the sampling procedures are presented. We review the recent progress in metabolomics approaches and a summary of their applications on the respiratory and non-respiratory diseases investigated by breath analysis. Recent reports on breathomics studies retrieved from Scopus and Pubmed were reviewed in this work. We conclude that analyzing breath metabolites (both volatile and nonvolatile) is valuable in disease diagnoses, and therefore believe that breathomics will turn into a promising noninvasive discipline in biomarker discovery and early disease detection in personalized medicine. The problem of wide variations in the reported metabolite concentrations from breathomics studies should be tackled by developing more accurate analytical methods and sophisticated numerical analytical alogorithms.

Automated summary

Metabolomics research, particularly in breathomics, is advancing rapidly in disease diagnosis, focusing on the collection and analysis methods of exhaled breath. Analyzing metabolites in exhaled breath is valuable for disease diagnosis, indicating the promising future of breathomics as a noninvasive discipline. Wide variations in reported metabolite concentrations from breathomics studies should be addressed with more accurate analytical methods and sophisticated numerical algorithms.