期刊
CLINICAL TOXICOLOGY
卷 59, 期 9, 页码 801-809出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/15563650.2020.1870691
关键词
CNS; psychological; organ; tissue specific; complications of poisoning; metabolic; complications of poisoning; other
类别
资金
- National Institutes of Health-NIH [K08HL136858, R21ES031243, R03HL154232, R01HL141386]
This study developed a porcine model of CO poisoning and found that low-dose CO poisoning led to decreased mitochondrial respiration and increased ROS generation in the brain and heart tissues. Additionally, there was an increase in cerebral lactate-to-pyruvate ratio and plasma cytokine concentrations in the CO group. These results suggest that mitochondrial dysfunction plays a crucial role in the pathology of CO poisoning, representing a potential target for therapeutic interventions.
Objectives The purpose of this study is the development of a porcine model of carbon monoxide (CO) poisoning to investigate alterations in brain and heart mitochondrial function. Design Two group large animal model of CO poisoning. Setting Laboratory. Subjects Ten swine were divided into two groups: Control (n = 4) and CO (n = 6). Interventions Administration of a low dose of CO at 200 ppm to the CO group over 90 min followed by 30 min of re-oxygenation at room air. The Control group received room air for 120 min. Measurements Non-invasive optical monitoring was used to measure cerebral blood flow and oxygenation. Cerebral microdialysis was performed to obtain semi real time measurements of cerebral metabolic status. At the end of the exposure, both fresh brain (cortical and hippocampal tissue) and heart (apical tissue) were immediately harvested to measure mitochondrial respiration and reactive oxygen species (ROS) generation and blood was collected to assess plasma cytokine concentrations. Main results Animals in the CO group showed significantly decreased Complex IV-linked mitochondrial respiration in hippocampal and apical heart tissue but not cortical tissue. There also was a significant increase in mitochondrial ROS generation across all measured tissue types. The CO group showed a significantly higher cerebral lactate-to-pyruvate ratio. Both IL-8 and TNF alpha were significantly increased in the CO group compared with the Control group obtained from plasma. While not significant there was a trend to an increase in optically measured cerebral blood flow and hemoglobin concentration in the CO group. Conclusions Low-dose CO poisoning is associated with early mitochondrial disruption prior to an observable phenotype highlighting the important role of mitochondrial function in the pathology of CO poisoning. This may represent an important intervenable pathway for therapy and intervention.
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