期刊
CHINESE CHEMICAL LETTERS
卷 32, 期 10, 页码 3185-3188出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2021.03.012
关键词
Bird nest-like zinc oxide; Nanostructure; Glucose oxidase; Electrochemical; Biosensor
资金
- National Natural Science Foundation of China [21575125, 21475116]
- Natural Science Foundation of Jiangsu Province [BK20191434]
- Project for Science and Technology of Yangzhou [YZ2020068]
- Project for Science and Technology of Zhenjiang [GY2020028]
- Zhejiang Provincial Natural Science Foundation of China [LY20B050008]
- Zhejiang Provincial Project of Medical and Health Technology [2021RC139]
- Key Project of Social Development of Jinhua [2020-3-033]
- 333 Project
- Qinglan Project of Jiangsu Province
- high-end talent support program of Yangzhou University
- Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD)
- Six Talent Peaks Project of Jiangsu Province
A novel bird nest-like zinc oxide (BN-ZnO) nanostructures were prepared and used to develop a sensitive electrochemical glucose biosensor. The biosensor showed high sensitivity and a wide linear range, benefitting from the large specific surface area of BN-ZnO and its ability to retain the native bioactivity of enzymes.
In this research, a novel bird nest-like zinc oxide (BN-ZnO) nanostructures were prepared by a simple solvothermal method. A sensitive electrochemical glucose biosensor was for the first time developed based on the immobilization of glucose oxidase (GOx) on nanostructured BN-ZnO modified electrode. The BN-ZnO nanostructure and the resultant biosensor were characterized by scanning electron microscope, X-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, and electrochemical impedance spectroscopy. BN-ZnO nanostructures have large specific surface area and can load large amounts of GOx molecules. Meanwhile, BN-ZnO provides an excellent microenvironment to retain the native bioactivity of enzymes and to promote direct electron transfer between GOx and electrode surface. The proposed biosensor shows a wide linear range of 0.005-1.6 mmol/L, high sensitivity of 15.6 mA L mol(-1) cm(-2) with a low detection limit of 0.004 mmol/L. The resulting biosensor also shows excellent selectivity, acceptable stability and reproducibility, and can be successfully applied in the detection of glucose in human serum samples at -0.37 V. (C) 2021 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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