期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 27, 期 27, 页码 7533-7541出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.202100328
关键词
lipid bilayers; membrane modulation; neurotransmission; serotonin-membrane interaction; volume transmission
资金
- Department of Atomic Energy, Government of India [RTI4003]
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [SFB 1423, 421152132]
- Sigrid Juselius Foundation
- Ruth and Nils-Erik Stenback's Foundation
- Projekt DEAL
Serotonin can modulate membrane properties and cellular function by directly binding to the membrane, independent of receptor activity. This interaction potentiates key cellular processes, such as transferrin receptor endocytosis, in a receptor-independent fashion.
Serotonin, an important signaling molecule in humans, has an unexpectedly high lipid membrane affinity. The significance of this finding has evoked considerable speculation. Here we show that membrane binding by serotonin can directly modulate membrane properties and cellular function, providing an activity pathway completely independent of serotonin receptors. Atomic force microscopy shows that serotonin makes artificial lipid bilayers softer, and induces nucleation of liquid disordered domains inside the raft-like liquid-ordered domains. Solid-state NMR spectroscopy corroborates this data at the atomic level, revealing a homogeneous decrease in the order parameter of the lipid chains in the presence of serotonin. In the RN46A immortalized serotonergic neuronal cell line, extracellular serotonin enhances transferrin receptor endocytosis, even in the presence of broad-spectrum serotonin receptor and transporter inhibitors. Similarly, it increases the membrane binding and internalization of oligomeric peptides. Our results uncover a mode of serotonin-membrane interaction that can potentiate key cellular processes in a receptor-independent fashion.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据