期刊
CELL
卷 184, 期 4, 页码 899-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2021.01.003
关键词
-
资金
- NSF DDIG [DEB-1600920]
- Children's Orthopedic Research Foundation
Changes in appendage structure are crucial in vertebrate evolution, including the transition from fins to limbs. Zebrafish mutants with supernumerary long bones in their pectoral fins were identified, caused by activating mutations in appendage patterning regulators vav2 and waslb. This pathway, required for appendage development across vertebrates, drives enhanced expression of hoxa11b and reveals a limb-like pattern ability in fins.
Changes in appendage structure underlie key transitions in vertebrate evolution. Addition of skeletal elements along the proximal-distal axis facilitated critical transformations, including the fin-to-limb transition that permitted generation of diverse modes of locomotion. Here, we identify zebrafish mutants that form supernumerary long bones in their pectoral fins. These new bones integrate into musculature, form joints, and articulate with neighboring elements. This phenotype is caused by activating mutations in previously unrecognized regulators of appendage patterning, vav2 and waslb, that function in a common pathway. This pathway is required for appendage development across vertebrates, and loss of Wasl in mice causes defects similar to those seen in murine Hox mutants. Concordantly, formation of supernumerary bones requires Hox11 function, and mutations in the vav2/wasl pathway drive enhanced expression of hoxa11b, indicating developmental homology with the forearm. Our findings reveal a latent, limb-like pattern ability in fins that is activated by simple genetic perturbation.
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