期刊
CATALYSIS LETTERS
卷 151, 期 10, 页码 2996-3003出版社
SPRINGER
DOI: 10.1007/s10562-021-03535-6
关键词
Biogenic amine; Aromatic amino acid decarboxylase; Product inhibition; Ion exchange resin; In situ product removal
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [2019R1F1A1062845]
- Initiative for Biological Function & Systems under the BK21 PLUS Program of Korean Ministry of Education
- National Research Foundation of Korea [2019R1F1A1062845] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A novel biocatalytic process using AADC was developed for the preparative-scale production of biogenic amines, with broad substrate specificity and high conversion rates achieved under an ISPR strategy. The implementation of an open reactor system and cation exchange resin enabled efficient removal of reaction products, leading to high conversions within short reaction times.
Biogenic amines are important messengers in neurotransmission, drawing attention to pharmaceutical applications. Here, we developed a novel biocatalytic process for the preparation of biogenic amines using aromatic l-amino acid decarboxylase (AADC) under an in situ product removal (ISPR) condition. The AADC from Bacillus atrophaeus (AADC-BA) showed broad substrate specificity for aromatic amino acids, affording > 90% conversions of 10 mM substrates to corresponding products: 2-phenylethylamine (2-PEA), tryptamine, serotonin, and dopamine. Owing to enzyme inhibitions by the reaction products, we implemented an ISPR strategy using a cation exchange resin and an open reactor system for removal of amine and CO2, respectively. Under the open-air conditions, the addition of Dowex 50WX8 afforded 81% conversion of 100 mM l-phenylalanine to 2-PEA within 1 h, whereas 56% conversion was obtained without the resin. The ISPR strategy enabled the preparative-scale production of serotonin from 5-hydroxy-l-tryptophan (405 mg), yielding > 99% conversion within 40 min. Graphic
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