期刊
CARBOHYDRATE POLYMERS
卷 255, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.117376
关键词
Alginate; Nanoparticles; Ascorbic acid; Colorimetry; Vitamin C; Fruit juice
资金
- Prince of Songkla University
A sensitive detection strategy for ascorbic acid using sodium alginate reduced/stabilized gold nanoparticles as the optical probe was successfully developed. This method showed good linearity for AA quantitation in vitamin C injection and fruit juice, with satisfactory accuracy and precision.
A sensitive detection strategy for ascorbic acid (AA), using sodium alginate reduced/stabilized gold nanoparticles (SA-AuNPs) as the optical probe, is reported. The SA-AuNPs were prepared by mixing gold salt and SA under stirring for 2 h at room temperature, without any further steps. The mixture was aged at 4 degrees C overnight, after which a faint-purple colloidal solution of SA-AuNPs was obtained. Characterization shows that the synthesis is incapable of reducing all Au3+ to Au degrees, but rather to mixture of Au degrees/Au+. The addition of AA to the SA-AuNPs probe reduced completely all Au+ to new AuNPs which were deposited on the pre-formed SA-AuNPs seed, leading to size increment and absorption spectra enhancement. The assay exhibited a good linearity between 12.5 and 150.0 mu M AA and low limit of quantification of 11.2 mu M. It was further used for AA quantitation in vitamin C injection and fruit juice with satisfactory accuracy and precision.
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