期刊
EMBO REPORTS
卷 17, 期 9, 页码 1281-1291出版社
WILEY-BLACKWELL
DOI: 10.15252/embr.201642282
关键词
colonization resistance; enterotoxigenic Bacteroides fragilis; in vivo strain competition; probiotics; type VI secretion
资金
- Department of Pediatrics at the University of Chicago
- Digestive Diseases Research Core Center at the University of Chicago [NIDDK P30DK42086]
- Burroughs Wellcome Foundation Investigators in the Pathogenesis of Infectious Disease Fellowship
- National Institutes of Health Medical Scientist Training Program at the University of Chicago [GM007281]
The microbiota is a major source of protection against intestinal pathogens; however, the specific bacteria and underlying mechanisms involved are not well understood. As a model of this interaction, we sought to determine whether colonization of the murine host with symbiotic non-toxigenic Bacteroides fragilis could limit acquisition of pathogenic enterotoxigenic B. fragilis. We observed strain-specific competition with toxigenic B. fragilis, dependent upon type VI secretion, identifying an effector-immunity pair that confers pathogen exclusion. Resistance against host acquisition of a second non-toxigenic strain was also uncovered, revealing a broader function of type VI secretion systems in determining microbiota composition. The competitive exclusion of enterotoxigenic B. fragilis by a non-toxigenic strain limited toxin exposure and protected the host against intestinal inflammatory disease. Our studies demonstrate a novel role of type VI secretion systems in colonization resistance against a pathogen. This understanding of bacterial competition may be utilized to define a molecularly targeted probiotic strategy.
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