期刊
EMBO REPORTS
卷 17, 期 2, 页码 167-177出版社
WILEY-BLACKWELL
DOI: 10.15252/embr.201540789
关键词
cis-prenyltransferase; dolichol; glycosylation; NgBR; vascular development
资金
- National Institutes of Health [R01 HL64793, R01 HL61371, R01 HL081190]
- American Heart Association
NgBR is a transmembrane protein identified as a Nogo-B-interacting protein and recently has been shown to be a subunit required for cis-prenyltransferase (cisPTase) activity. To investigate the integrated role of NgBR in vascular development, we have characterized endothelial-specific NgBR knockout embryos. Here, we show that endothelial-specific NgBR knockout results in embryonic lethality due to vascular development defects in yolk sac and embryo proper. Loss of NgBR in endothelial cells reduces proliferation and promotes apoptosis of the cells largely through defects in the glycosylation of key endothelial proteins including VEGFR2, VE-cadherin, and CD31, and defective glycosylation can be rescued by treatment with the end product of cisPTase activity, dolichol phosphate. Moreover, NgBR functions in endothelial cells during embryogenesis are Nogo-B independent. These data uniquely show the importance of NgBR and protein glycosylation during vascular development.
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