期刊
EMBO JOURNAL
卷 35, 期 20, 页码 2179-2191出版社
WILEY
DOI: 10.15252/embj.201694857
关键词
DIS3L2; ncRNAs; RNA surveillance; TSSa; uridylation
资金
- Wellcome Trust [084316/B/07/Z]
- Czech Science Foundation [16-21341S, 305/11/1095]
- Ministry of Education, Youth and Sports of the Czech Republic under the project CEITEC [LQ1601]
- Wellcome Trust [084316/B/07/Z] Funding Source: Wellcome Trust
Uridylation of various cellular RNA species at the 3' end has been generally linked to RNA degradation. In mammals, uridylated pre-let-7 miRNAs and mRNAs are targeted by the 3' to 5' exoribonuclease DIS3L2. Mutations in DIS3L2 have been associated with Perlman syndrome and with Wilms tumor susceptibility. Using in vivo cross-linking and immunoprecipitation (CLIP) method, we discovered the DIS3L2-dependent cytoplasmic uridylome of human cells. We found a broad spectrum of uridylated RNAs including rRNAs, snRNAs, snoRNAs, tRNAs, vault, 7SL, Y RNAs, mRNAs, lncRNAs, and transcripts from pseudogenes. The unifying features of most of these identified RNAs are aberrant processing and the presence of stable secondary structures. Most importantly, we demonstrate that uridylation mediates DIS3L2 degradation of short RNA polymerase II-derived RNAs. Our findings establish the role of DIS3L2 and oligouridylation as the cytoplasmic quality control for highly structured ncRNAs.
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