期刊
EMBO JOURNAL
卷 35, 期 3, 页码 302-318出版社
WILEY-BLACKWELL
DOI: 10.15252/embj.201592929
关键词
dendrite; doublecortin; kinesin; neuron; polarity
资金
- Netherland Organization for Scientific Research (NWO-ALW-VIDI)
- Netherland Organization for Scientific Research (NWO-ALW-VICI)
- Foundation for Fundamental Research on Matter (FOM), NWO
- Netherlands Organization for Health Research and Development (ZonMW-TOP)
- European Research Council (ERC)
- Foundation for Polish Science
- European Union Regional Development Fund
- Polish National Science Centre Sonata Bis grant [2012/07/E/NZ3/00503]
- Foundation for Polish Science Mistrz
In neurons, the polarized distribution of vesicles and other cellular materials is established through molecular motors that steer selective transport between axons and dendrites. It is currently unclear whether interactions between kinesin motors and microtubule-binding proteins can steer polarized transport. By screening all 45 kinesin family members, we systematically addressed which kinesin motors can translocate cargo in living cells and drive polarized transport in hippocampal neurons. While the majority of kinesin motors transport cargo selectively into axons, we identified five members of the kinesin-3 (KIF1) and kinesin-4 (KIF21) subfamily that can also target dendrites. We found that microtubule-binding protein doublecortin-like kinase 1 (DCLK1) labels a subset of dendritic microtubules and is required for KIF1-dependent dense-core vesicles (DCVs) trafficking into dendrites and dendrite development. Our study demonstrates that microtubule-binding proteins can provide local signals for specific kinesin motors to drive polarized cargo transport.
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