期刊
EMBO JOURNAL
卷 35, 期 22, 页码 2468-2483出版社
WILEY
DOI: 10.15252/embj.201694248
关键词
MAPKKK5; PAMP; plant immunity; RLCK; signal transduction
资金
- Strategic Project to Support the Formation of Research Bases at Private Universities: Matching Fund Subsidy from the Ministry of Education, Culture, Sports, Science and Technology [S1101035]
- Basic Science Research Projects from Sumitomo Foundation
- Plant Global Education Project of Nara Institute of Science and Technology
- Max Planck Society
- Deutsche Forschungsgemeinschaft (DFG) [SFB 670]
- [JP15H02489]
- [JP15H01242]
- [JP16H01474]
- [JP15K18649]
- Grants-in-Aid for Scientific Research [15H01242, 15J09701, 15K18649, 14J07373, 15H05959, 16H01474, 15H01247, 15H02489] Funding Source: KAKEN
Perception of microbe-associated molecular patterns by host cell surface pattern recognition receptors (PRRs) triggers the intracellular activation of mitogen-activated protein kinase (MAPK) cascades. However, it is not known how PRRs transmit immune signals to MAPK cascades in plants. Here, we identify a complete phospho-signaling transduction pathway from PRR-mediated pathogen recognition to MAPK activation in plants. We found that the receptor-like cytoplasmic kinase PBL27 connects the chitin receptor complex CERK1-LYK5 and a MAPK cascade. PBL27 interacts with both CERK1 and the MAPK kinase kinase MAPKKK5 at the plasma membrane. Knockout mutants of MAPKKK5 compromise chitin-induced MAPK activation and disease resistance to Alternaria brassicicola. PBL27 phosphorylates MAPKKK5 invitro, which is enhanced by phosphorylation of PBL27 by CERK1. The chitin perception induces disassociation between PBL27 and MAPKKK5 invivo. Furthermore, genetic evidence suggests that phosphorylation of MAPKKK5 by PBL27 is essential for chitin-induced MAPK activation in plants. These data indicate that PBL27 is the MAPKKK kinase that provides the missing link between the cell surface chitin receptor and the intracellular MAPK cascade in plants.
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