期刊
EMBO JOURNAL
卷 35, 期 22, 页码 2435-2446出版社
WILEY
DOI: 10.15252/embj.201695020
关键词
ChEC-seq; Mediator; TFIID
资金
- NIH [R01GM053451, R01GM075114]
- Howard Hughes Medical Institute
- Indiana University startup funds
Mediator is a conserved, essential transcriptional coactivator complex, but its invivo functions have remained unclear due to conflicting data regarding its genome-wide binding pattern obtained by genome-wide ChIP. Here, we used ChEC-seq, a method orthogonal to ChIP, to generate a high-resolution map of Mediator binding to the yeast genome. We find that Mediator associates with upstream activating sequences (UASs) rather than the core promoter or gene body under all conditions tested. Mediator occupancy is surprisingly correlated with transcription levels at only a small fraction of genes. Using the same approach to map TFIID, we find that TFIID is associated with both TFIID- and SAGA-dependent genes and that TFIID and Mediator occupancy is cooperative. Our results clarify Mediator recruitment and binding to the genome, showing that Mediator binding to UASs is widespread, partially uncoupled from transcription, and mediated in part by TFIID.
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