期刊
BRAIN PATHOLOGY
卷 31, 期 3, 页码 -出版社
WILEY
DOI: 10.1111/bpa.12936
关键词
Alzheimer‐ type synaptopathogenesis; mild traumatic brain injury; NBDP; NCAM breakdown products; neurotrauma; synaptic decline
资金
- National Institutes of Health [5R25GM077634]
- Intramural Research Program of the National Institute of Environmental Health Sciences, National Institutes of Health [Z01 ES100221]
- U.S. Army Research Laboratory
- U.S. Army Research Office [W911NF-14-2-0087]
Explosive shockwaves and blast exposures are associated with injuries common in military service and increased risk for dementia. Blast-induced changes in neuronal circuitries were identified, with explosions leading to reductions in synaptic markers linked to cognitive disorders without causing neuronal loss.
Explosive shockwaves, and other types of blast exposures, are linked to injuries commonly associated with military service and to an increased risk for the onset of dementia. Neurological complications following a blast injury, including depression, anxiety, and memory problems, often persist even when brain damage is undetectable. Here, hippocampal explants were exposed to the explosive 1,3,5-trinitro-1,3,5-triazinane (RDX) to identify indicators of blast-induced changes within important neuronal circuitries. Highly controlled detonations of small, 1.7-gram RDX spherical charges reduced synaptic markers known to be downregulated in cognitive disorders, but without causing overt neuronal loss or astroglial responses. In the absence of neuromorphological alterations, levels of synaptophysin, GluA1, and synapsin IIb were significantly diminished within 24 hr, and these synaptic components exhibited progressive reductions following blast exposure as compared to their stable maintenance in control explants. In contrast, labeling of the synapsin IIa isoform remained unaltered, while neuropilar staining of other markers decreased, including synapsin IIb and neural cell adhesion molecule (NCAM) isoforms, along with evidence of NCAM proteolytic breakdown. NCAM(180) displayed a distinct decline after the RDX blasts, whereas NCAM(140) and NCAM(120) exhibited smaller or no deterioration, respectively. Interestingly, the extent of synaptic marker reduction correlated with AT8-positive tau levels, with tau pathology stochastically found in CA1 neurons and their dendrites. The decline in synaptic components was also reflected in the size of evoked postsynaptic currents recorded from CA1 pyramidals, which exhibited a severe and selective reduction. The identified indicators of blast-mediated synaptopathy point to the need for early biomarkers of explosives altering synaptic integrity with links to dementia risk, to advance strategies for both cognitive health and therapeutic monitoring.
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