Streptozotocin induces alpha-2u globulin nephropathy in male rats during diabetic kidney disease

BackgroundAlpha-2u globulin nephropathy mainly shows toxicological pathology only in male rats induced by certain chemicals and drugs, such as levamisole (antiparasitic and anticancer drugs). Streptozotocin (STZ) is also an anticancer-antibiotic agent that has been used for decades to induce a diabetic kidney disease model in rodents. The purpose of this study is to determine if STZ causes alpha-2u globulin nephropathy in male rats during an advanced stage of diabetic kidney disease. Alpha-2u globulin nephropathy, water absorption and filtration capacities (via aquaporin [AQP]-1, -2, -4 and-5) and mitochondrial function (through haloacid dehalogenase-like hydrolase domain-containing protein [HDHD]-3 and NADH-ubiquinone oxidoreductase 75kDa subunit [NDUFS]-1 proteins) were examined in STZ-induced diabetic Wistar rat model.ResultsMore than 80% of severe clinical illness rats induced by STZ injection simultaneously exhibited alpha-2u globulin nephropathy with mitochondrial degeneration and filtration apparatus especially pedicels impairment. They also showed significantly upregulated AQP-1, -2, -4 and-5, HDHD-3 and NDUFS-1 compared with those of the rats without alpha-2u globulin nephropathy.ConclusionsSTZ-induced alpha-2u globulin nephropathy during diabetic kidney disease in association with deterioration of pedicels, renal tubular damage with adaptation and mitochondrial driven apoptosis.

Automated summary

More than 80% of severely ill rats induced by STZ injections simultaneously exhibited alpha-2u globulin nephropathy with mitochondrial degeneration and impairment of the filtration apparatus, especially the pedicels. Additionally, these rats showed significantly upregulated levels of AQP-1, -2, -4 and-5, HDHD-3, and NDUFS-1 compared to those without alpha-2u globulin nephropathy.