期刊
BIOTECHNOLOGY LETTERS
卷 43, 期 4, 页码 919-932出版社
SPRINGER
DOI: 10.1007/s10529-021-03083-4
关键词
Salmonella; Label-free quantitative proteomics; YggT; Antibiotic resistance
资金
- National Natural Science Foundation of China [31802246]
- Science and Technology Program of Guangzhou [201804010207]
Proteomic and mutagenic analyses revealed that yggT is involved in streptomycin resistance in Salmonella enterica D14.
Objectives To identify proteins that may be associated with antibiotic resistance in the multidrug-resistant Salmonella enterica D14, by constructing proteomic profiles using mass spectrometry-based label-free quantitative proteomics (LFQP). Results D14 was cultured with four antibiotics (ampicillin, nalidixic acid, streptomycin, and tetracycline) separately. Subsequently, the findings from an equal combination of the four cultures were compared with the profile of sensitive S. enterica 104. 2255 proteins, including 149 differentially up-regulated proteins, were identified. Many of these up-regulated proteins were associated with flagellar assembly and chemotaxis, two-component system, amino acid metabolism, beta-lactam resistance, and transmembrane transport. A subset of 10 genes was evaluated via quantitative real-time PCR (qPCR), followed by the construction of cheR, fliS, fliA, arnA, and yggT deletion mutants. Only the yggT-deleted D14 mutant showed decrease in streptomycin resistance, whereas the other deletions had no effect. Furthermore, complementation of yggT and the overexpression of yggT in S. enterica ATCC 14028 increased the streptomycin resistance. Additionally, spot dilution assay results confirmed that Salmonella strains, harboring yggT, exhibited an advantage in the presence of streptomycin. Conclusions The above proteomic and mutagenic analyses revealed that yggT is involved in streptomycin resistance in S. enterica.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据