期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 118, 期 5, 页码 1862-1875出版社
WILEY
DOI: 10.1002/bit.27702
关键词
biomimetic; bone regeneration; eggshell membrane; nanotopography; tissue engineering
资金
- National Research Foundation of Korea [2016M3A9B4919374, 2019M3A9H1103737, 2019R1I1A3A0106345]
- Agricultural Robotics and Automation Research Center through the Agriculture, Food and Rural Affairs Research Center Support Program, Ministry of Agriculture, Food and Rural Affairs. [714002-7]
- National Research Foundation of Korea [2016M3A9B4919374, 2019M3A9H1103737] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The study developed a polycaprolactone-based scaffold for bone regeneration with a soluble eggshell membrane protein coating and nanotopography structure, exhibiting flexibility and mechanical strength. In vitro experiments showed that the scaffold could regulate and enhance cell morphology, adhesion, proliferation, and differentiation.
A bone regeneration scaffold is typically designed as a platform to effectively heal a bone defect while preventing soft tissue infiltration. Despite the wide variety of scaffold materials currently available, such as collagen, critical problems in achieving bone regeneration remain, including a rapid absorption period and low tensile strength as well as high costs. Inspired by extracellular matrix protein and topographical cues, we developed a polycaprolactone-based scaffold for bone regeneration using a soluble eggshell membrane protein (SEP) coating and a nanotopography structure for enhancing the physical properties and bioactivity. The scaffold exhibited adequate flexibility and mechanical strength as a biomedical platform for bone regeneration. The highly aligned nanostructures and SEP coating were found to regulate and enhance cell morphology, adhesion, proliferation, and differentiation in vitro. In a calvaria bone defect mouse model, the scaffolds coated with SEP applied to the defect site promoted bone regeneration along the direction of the nanotopography in vivo. These findings demonstrate that bone-inspired nanostructures and SEP coatings have high potential to be applicable in the design and manipulation of scaffolds for bone regeneration.
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