期刊
BIOSENSORS & BIOELECTRONICS
卷 174, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112825
关键词
Surface-enhanced Raman scattering; Raman reporter indicator mechanism; Capillary sensor; Mussel-inspired molecular imprinting; Bioassay
类别
资金
- Chinese Academy of Sciences President's International Fellowship Initiative [2019PC0050, 2020PC0083]
- National Natural Science Foundation of China [42076199, 21976209, 21804010, 22006162, 21976099, 81573393]
- Science and Technology Development Plan of Shandong Province of China [2019GSF108047]
- Youth Innovation Promotion Association CAS [2017256]
- Instrument Developing Project of the Chinese Academy of Sciences [YZ201662]
- Taishan Scholar Project Special Funding [Ts20190962]
- Technical Innovation Project of Instrument Function Development of the Chinese Academy of Sciences
A capillary sensor was developed for ultrasensitive and universal detection of protein biomarkers using an inner SERS substrate layer and an outer imprinted layer. The imprinted cavities selectively captured target proteins and allowed passage of Raman indicator to access the SERS substrate, leading to specific and reproducible detection of trypsin enzyme in biological samples without sample preparation. The platform offers a facile, fast, and general route for sensitive SERS detection of Raman inactive biomacromolecules.
It is still challenging to sensitively detect protein biomarkers via surface-enhanced Raman scattering (SERS) technique owing to their low Raman activity. SERS tag-based immunoassay is usually applied; however, it is laborious and needs specific antibodies. Herein, an ultrasensitive and universal Raman indicator sensing strategy is proposed for protein biomarkers, with the aid of a glass capillary-based molecularly imprinted SERS sensor. The sensor consists of an inner SERS substrate layer for signal enhancement and an outer mussel-inspired polydopamine imprinted layer as a recognition element. Imprinted cavities have two missions: first, selectively capturing the target protein, and second, the only passageway of Raman indicator to access SERS substrate. Specific protein recognition means filling imprinted cavities and blocking Raman indicator flow. Thus, the quantity of captured protein can be reflected by the signal decrease of ultra-Raman active indicator molecule. The capillary sensor exhibited specific and reproducible detection at the level down to 4.1 x 10(-3) mu g L-1, for trypsin enzyme in as-received biological samples without sample preparation. The generality of the mechanism is confirmed by using three different protein models. This platform provides a facile, fast and general route for sensitive SERS detection of Raman inactive biomacromolecules, which offers great promising utility for in situ and fast point-of-care practical bioassay.
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