期刊
BIOSENSORS & BIOELECTRONICS
卷 176, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112953
关键词
Bacterial diagnostic; SARS-CoV-2; Botulinum toxin; Fluorescence resonance energy transfer; Colorimetric detection; Biosensors
类别
资金
- Key Research and the Development Program of Hainan, China [ZDYF(XGFY)2020002]
- National Key Research and Development Program of China, China [2018YFD0900704]
- National Natural Science Foundation of China, China [41866002]
- Tsinghua University Initiative Scientific Research Program, China [2020Z99CFZ019]
- Shandong Key Laboratory of Corrosion Science, China [KLCS201910]
- Research Foundation of Hainan University, China [KYQD(ZR)1711]
The detection strategy utilizes BoNT/A LC activated complex assay, nanoparticle hybridization system, and Fluorescence Resonance Energy Transfer technology to achieve high sensitivity in pathogenic microorganism detection, providing a simple instrument-free colorimetric tool for global public health emergencies.
The rapid identification of pathogenic microorganisms plays a crucial role in the timely diagnosis and treatment strategies during a global pandemic, especially in resource-limited area. Herein, we present a sensitive biosensor strategy depended on botulinum neurotoxin type A light chain (BoNT/A LC) activated complex assay (BACA). BoNT/A LC, the surrogate of BoNT/A which embodying the most potent biological poisons, could serve as an ultrasensitive signal reporter with high signal-to-noise ratio to avoid common strong background response, poor stability and low intensity of current biosensor methods. A nanoparticle hybridization system, involving specific binding probes that recognize pathogenic 16S rRNAs or SARS-CoV-2 gene site, was developed to measure doublestranded biotinylated target DNA containing a single-stranded overhang using Fluorescence Resonance Energy Transfer (FRET)-based assay and colorimetric method. The method is validated widely by six different bacteria strains and severe acute respiratory related coronavirus 2 (SARS-CoV-2) nucleic acid, demonstrating a single cell or 1 aM nucleic acid detecting sensitivity. This detection strategy offers a solution for general applications and has a great prospect to be a simple instrument-free colorimetric tool, especially when facing public health emergency.
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