期刊
BIOSENSORS & BIOELECTRONICS
卷 176, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112920
关键词
Coronavirus disease; Microfluidic chip; Immunoassay; Interaction dynamics; Multiplexed analysis
类别
资金
- National Natural Science Foundation of China [21775168]
- National Key R&D Program of China [2017YFE0102400]
- Fundamental Research Funds for the Central Universities [20lgzd28]
- Shenzhen Research Funding Program [JCYJ20180307155036127]
- Special Fund for New Coronavirus Prevention from Sun Yat-sen University
- COVID-19 Emerging Prevention Products, Research Special Fund of Zhuhai City [ZH22036302200016PWC]
The study introduced a reciprocating-flowing immunobinding (RFimmunobinding) strategy that achieves adequate immunobinding within 60 seconds. This strategy was further developed into an immunoassay method for the serological detection of 13 suspected COVID-19 patients, achieving a 100% true negative and true positive rate.
The worldwide epidemic of novel coronavirus disease (COVID-19) has led to a strong demand for highly efficient immunobinding to achieve rapid and accurate on-site detection of SARS-CoV-2 antibodies. However, hour-scale time-consumption is usually required to ensure the adequacy of immunobinding on expensive large instruments in hospitals, and the common false negative or positive results often occur in rapid on-site immunoassay (e.g. immunochromatography). We solved this dilemma by presenting a reciprocating-flowing immunobinding (RFimmunobinding) strategy. RF-immunobinding enabled the antibodies in fluid contacting with the corresponding immobilized antigens on substrate repeatedly during continuous reciprocating-flowing, to achieve adequate immunobinding within 60 s. This strategy was further developed into an immunoassay method for the serological detection of 13 suspected COVID-19 patients. We obtained a 100% true negative and true positive rate and a limit of quantification (LOQ) of 4.14 pg/mL. Our strategy also can be a potential support for other areas related to immunorecognition, such as proteomics, immunopharmacology and immunohistochemistry.
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