期刊
BIOSENSORS & BIOELECTRONICS
卷 176, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112907
关键词
Biofuel cells; Self-powered biosensor; GDH@ZIF-8; Nano-enrichment carriers; Exosomes bioassay
类别
资金
- National Natural Science Foundation of China [21605092, 21775082]
- Natural Science Foundation of Shandong Province [ZR2019YQ23]
- Major Program of Shandong Province Natural Science Foundation [ZR2018ZC0127]
- Research Foundation for Distinguished Scholars of Qingdao Agricultural University [663-1117002]
- Special Foundation for Taishan Scholar of Shandong Province [ts201511052]
- Shandong Province Higher Educational Program for Young Innovation Talents
In this study, a BFC-based self-powered biosensor with enhanced stability and sensitivity was successfully constructed using dual metal-organic frameworks (MOFs). The biosensor achieved high sensitivity and specificity in detecting exosomes, with a detection limit of 300 particles mL(-1). This approach shows great potential for expanding the applications of BFC-based self-powered biosensors.
Biofuel cells (BFCs)-based self-powered biosensors suffer from the limited stability of bioenzymes. Meanwhile, the poor performance of self-powered biosensors affects the sensitivity of biosensing, thus, it is significant and challenging to improve their stability and sensitivity. In our work, a BFC-based self-powered biosensor, with simultaneously enhanced stability and sensitivity, was constructed utilizing dual metal-organic frameworks (MOFs) as the carriers of the bioenzyme and the electroactive probe, respectively. Anodic enzyme, glucose dehydrogenase (GDH), was encapsulated in zeolitic imidazolate framework-8 (ZIF-8) to form GDH@ZIF-8 composites, enhancing the catalytic activity and stability of GDH. Meanwhile, another zirconium metal-organic frameworks (UiO-66-NH2) loaded with electroactive molecules (K3[Fe(CN)(6)]) served as nano-enrichment carriers and improved the capability of the cathode to accept electrons from the anode, further improving the sensitivity of the as-proposed biosensor. Herein, the signal-on BFC-based self-powered biosensing of exosomes, the model analyte, with excellent stability and outstanding sensitivity was realized with the assistance of dual MOFs, and the detection limit was down to 300 particles mL(-1) (based on 3s/k), which was superior to those previously reported in literatures. Furthermore, the developed protocol was capable of detecting exosomes derived from cancer cells in complex biological samples. Overall, in this work the enhancement of both stability and sensitivity has been achieved by utilizing two types of MOFs, which laid the foundation for expanding the applications of BFC-based self-powered biosensors.
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