期刊
BIOPHYSICAL CHEMISTRY
卷 270, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bpc.2020.106538
关键词
SARS-CoV-2; RT-PCR; Biosensors; Nucleic-acid amplification; CRISPR-Cas; Next-generation sequencing; Microarray; Immunosensor; Serological test
The global outbreak of COVID-19 due to SARS-CoV-2 has created an unprecedented humanitarian crisis, calling for a sensitive, specific, fast, and affordable diagnostic technique. Modern technologies such as CRISPR-based detection platforms or microarray kits have shown enhanced sensitivity and specificity, offering promising solutions for efficient disease management.
The current global outbreak of COVID-19 due to SARS-CoV-2 is an unprecedented humanitarian crisis. Considering the gravity of its impact there is an immediate need to develop a detection technique that is sensitive, specific, fast, and affordable for the clinical diagnosis of the disease. Real time Polymerase Chain Reaction (RT-PCR)-based detection platforms are contemplated to be the gold standard to detect viral RNA. However, that may be susceptible to errors, and there is a risk of obtaining false results, which ultimately compromises the strategy of efficient disease management. Several modern techniques exhibiting assured results with enhanced sensitivity and specificity against the SARS-CoV-2 associated viral components or immune response against it have been developed and may be implemented. The review deals with the conventional RT-PCR detection techniques and compares them to other detection platforms viz., biosensor based detection of antigens, fluorescent or colorimetric detection systems including CRISPR-Cas 13 based SHERLOCK kit, CRISPR Cas-9 based FELUDA test kit, CRISPR DETECTR kit, Next Generation Sequencing or microarray-based kits. These modern techniques are great as a point of care detection methods but should be followed by RT PCR based detection for the confirmation of COVID-19 status.
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