Synthesis and biological activities of novel mitochondria-targeted artemisinin ester derivatives

A series of novel artemisinin ester derivatives were designed and synthesized for targeting mitochondria. Cytotoxicity against SMMC-7721, HepG2, OVCAR3, A549 and J82 cancer cell lines was evaluated. Compound 2c (IC50 = 3.0 ?M) was the most potent anti-proliferative molecule against the OVCAR3 cells with low cytotoxicity in normal HUVEC cells. The mechanism of action of compound 2c was further investigated by analyzing cell apoptosis, mitochondrial membrane potential (??m) and intracellular ROS generation. The results indicated that compound 2c targeted mitochondria and induced cell apoptosis. ROS and heme attributed to the cytotoxicity and cell apoptosis of compound 2c. These promising findings indicated the compound 2c could serve as a great candidate against ovarian cancer for further investigation.

Automated summary

Novel artemisinin ester derivatives were synthesized and compound 2c showed the most potent anti-proliferative activity against OVCAR3 cells with low cytotoxicity, likely through targeting mitochondria and inducing cell apoptosis. These findings suggest compound 2c may be a promising candidate for further investigation against ovarian cancer.