期刊
BIOMEDICAL CHROMATOGRAPHY
卷 35, 期 7, 页码 -出版社
WILEY
DOI: 10.1002/bmc.5109
关键词
aptamer; capillary electrophoresis; screening; SELEX
类别
资金
- Natural Science Foundation of Jiangsu Province in China [BK20151338]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Oligonucleotide aptamers, as a new molecular recognition element, offer advantages such as high affinity and specificity to target molecules. The methodology for screening aptamers is continuously being improved, with capillary electrophoresis-based methods showing increased efficiency and success rates.
As a new molecular recognition element, oligonucleotide aptamer not only has higher affinity and specificity to target molecules, but also has the advantages of wide recognition range, in vitro synthesis and chemical stability compared with conventional antibodies. Since a kind of screening method termed systematic evolution of ligands by exponential enrichment (SELEX) was reported, scientists have extensively researched the methodology of how to highly and efficiently screen out aptamers from a library consisting of a large number of random oligonucleotides. Certainly capillary electrophoresis-based screening methodologies, including nonequilibrium capillary electrophoresis of equilibrium mixtures, equilibrium capillary electrophoresis of equilibrium mixtures, non-SELEX, ideal-filter capillary electrophoresis, capillary transient isotachophoresis, etc., are revolutionary. Compared with conventional SELEX, these capillary electrophoresis-based methodologies show incomparable advantages such as the single-round screening of aptamers and increased successful screening rate. Methodology studies on the screening process of aptamers are comprehensively reviewed.
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