期刊
BIOELECTROCHEMISTRY
卷 137, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2020.107643
关键词
Impedimetric immunosensor; 4,4 '-Thiobisbenzenethiol monolayer; Gold nano-particles; Half-antibody fragment; Amyloid-beta(1-42) fibrils
资金
- Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, Poland
This report presents an electrochemical immunosensor for monitoring A beta(1-42) fibrils, fabricated with stepwise assembly of the sensing platform and successfully applied in different media. The immunosensor shows better sensitivity in the presence of APP and moderate response towards A beta(1-42) oligomers, demonstrating good selectivity.
In this report, an electrochemical immunosensor for the selective and sensitive monitoring of A beta(1-42) fibrils is presented. The sensing platform was prepared by the formation of a 4,4 '-thiobisbenzenethiol (TBBT) self-assembled monolayer on a clean gold surface followed by the covalent entrapment of gold nanoparticles (AuNPs). The half-antibody fragments of the Anti-Amyloid Fibrils antibody were immobilized on AuNPs via S-Au covalent bonds. Each step of immunosensor fabrication was characterized with cyclic voltammetry and electrochemical impedance spectroscopy. The biosensor was successfully used for the sensing of A beta(1-42) fibrils in both phosphate saline buffer (PBS) and artificial blood plasma (ABP). The immunosensor sensitivity estimated based on calibration slopes was better in the presence of APP in the comparison to PBS. The LOD values obtained for both measuring media were of 0.6 pM level. The moderate response towards A beta(1-42) oligomers demonstrated the immunosensor selectivity. (C) 2020 Elsevier B.V. All rights reserved.
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