Nuclear transformation of Chlamydomonas reinhardtii: A review

Chlamydomonas reinhardtii is a model organism with three sequenced genomes capable of genetic transformation. C. reinhardtii has the advantages of being low cost, non-toxic, and having a posttranslational modification system that ensures the recombinant proteins have the same activity as natural proteins, thus making it a great platform for application in molecular biology and other fields. In this review, we summarize the existing methods for nuclear transformation of C. reinhardtii, genes for selection, examples of foreign protein expression, and factors affecting transformation efficiency, to provide insights into effective strategies for the nuclear transformation of C. reinhardtii. (C) 2020 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.

Automated summary

Chlamydomonas reinhardtii is a model organism with three sequenced genomes capable of genetic transformation, offering advantages of low cost, non-toxicity, and a posttranslational modification system ensuring recombinant proteins have the same activity as natural proteins. This review summarizes methods for nuclear transformation, genes for selection, foreign protein expression examples, and factors affecting transformation efficiency, providing insights for effective strategies.