期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 166, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bej.2020.107851
关键词
Hydrogenases; Succinate; Yield; Escherichia coli
资金
- National Key Research and Development Program of China [2018YFA0901300]
- Key Research Program of the Chinese Academy of Science [KFZD-SW-215]
- National Natural Science Foundation of China [31522002, 31770105]
By investigating the endogenous hydrogenase genes of Escherichia coli, a versatile biological platform was constructed for efficient utilization of H-2, with further improvements made through modulation of gene expression and engineering of the rTCA cycle and CO2 supplementation pathways, resulting in increased succinate yield.
H-2 is extensively available from geological reservoirs, industrial byproducts or natural processes, which makes H-2 an ideal supplemental substrate providing additional reductive equivalents for increasing anaerobic fermentation efficiency. While it has been applied broadly in industrial and pharmaceutical fileds, Escherichia coli was not shown to efficiently utilize hydrogen. To construct a versatile biological platform for efficient utilization of H-2, the endogenous hydrogenase genes of E. coli were investigated. Consequently, overexpression of the endogenous hydrogenases Hyd-1 and Hyd-2 induced respective 26.5 % and 36.7 % increases of succinate yield, indicating that both Hyd-1 and Hyd-2 contributed to the increased pool of reductive equivalents. Additionally, simultaneously modulating the expression of hya and hyb genes by introducing the strong promoter P10 resulted in the higher yield of succinate. To further improve the utilization of H-2, pathway of rTCA cycle and CO2 supplementation were engineered in the strain ZL014(pBTCA), which produced the highest succinate yield of 1.50 mol per mol glucose. Our work successfully engineered a novel E. coli platform that could efficiently utilize H-2, which could be employed to construct a variety of microbial cell factories requiring substantial amounts of reductive equivalents.
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