期刊
BASIC RESEARCH IN CARDIOLOGY
卷 116, 期 1, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00395-021-00846-y
关键词
Cardiac fibrosis; Cardiac fibroblast; Myofibroblast; AMPKα 1; Connexin 43; miR-125b-5p
资金
- Fonds National de la Recherche Scientifique et Medicale (FNRS, Belgium)
- Action de Recherche Concertee de la Communaute Wallonie-Bruxelles, Belgium [ARC 13/18-051, ARC 16/21-074]
- Astra Zeneca
- FRIA fellowship (FNRS, Belgium)
- Bourse du Patrimoine (UCL, Belgium)
- European Society of Cardiology (ESC) Research Grant
- Fonds de Recherche Clinique (Cliniques Universitaires SaintLuc, Belgium)
- National Institutes of Health [HL135657, HL148165]
Deleting AMPKα1 in myofibroblasts exacerbates adverse LV remodeling post-MI, enhances fibrotic response, and affects Cx43 expression, potentially through transcriptional and post-transcriptional regulation involving miR-125b-5p.
We have previously demonstrated that systemic AMP-activated protein kinase alpha 1 (AMPK alpha 1) invalidation enhanced adverse LV remodelling by increasing fibroblast proliferation, while myodifferentiation and scar maturation were impaired. We thus hypothesised that fibroblastic AMPK alpha 1 was a key signalling element in regulating fibrosis in the infarcted myocardium and an attractive target for therapeutic intervention. The present study investigates the effects of myofibroblast (MF)-specific deletion of AMPK alpha 1 on left ventricular (LV) adaptation following myocardial infarction (MI), and the underlying molecular mechanisms. MF-restricted AMPK alpha 1 conditional knockout (cKO) mice were subjected to permanent ligation of the left anterior descending coronary artery. cKO hearts exhibit exacerbated post-MI adverse LV remodelling and are characterised by exaggerated fibrotic response, compared to wild-type (WT) hearts. Cardiac fibroblast proliferation and MF content significantly increase in cKO infarcted hearts, coincident with a significant reduction of connexin 43 (Cx43) expression in MFs. Mechanistically, AMPK alpha 1 influences Cx43 expression by both a transcriptional and a post-transcriptional mechanism involving miR-125b-5p. Collectively, our data demonstrate that MF-AMPK alpha 1 functions as a master regulator of cardiac fibrosis and remodelling and might constitute a novel potential target for pharmacological anti-fibrotic applications.
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