期刊
ARCHIVES OF TOXICOLOGY
卷 95, 期 5, 页码 1763-1778出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00204-021-03016-0
关键词
Cigarette smoke; Human air– liquid-interface (ALI) airway tissue model; Transcriptome analysis; MicroRNA; Cilia
类别
资金
- U.S. Food and Drug Administration Center for Tobacco Products (CTP)
Exposure to cigarette smoke has been shown to impair mucociliary clearance and is associated with chronic obstructive pulmonary diseases. In this study, miRNAs associated with impaired mucociliary clearance in an in vitro airway tissue model exposed to cigarette smoke were identified. The downregulation of miR-449a by cigarette smoke may interfere with ciliogenesis and derepress cell-cycle proteins, potentially serving as an early biomarker for cigarette smoke exposure and harm.
Exposure to cigarette smoke (CS) is strongly associated with impaired mucociliary clearance (MCC), which has been implicated in the pathogenesis of CS-induced respiratory diseases, such as chronic obstructive pulmonary diseases (COPD). In this study, we aimed to identify microRNAs (miRNAs) that are associated with impaired MCC caused by CS in an in vitro human air-liquid-interface (ALI) airway tissue model. ALI cultures were exposed to CS (diluted with 0.5 L/min, 1.0 L/min, and 4.0 L/min of clean air) from smoking five 3R4F University of Kentucky reference cigarettes under the International Organization for Standardization (ISO) machine smoking regimen, every other day for 1 week (a total of 3 days, 40 min/day). Transcriptome analyses of ALI cultures exposed to the high concentration of CS identified 5090 differentially expressed genes and 551 differentially expressed miRNAs after the third exposure. Genes involved in ciliary function and ciliogenesis were significantly perturbed by repeated CS exposures, leading to changes in cilia beating frequency and ciliary protein expression. In particular, a time-dependent decrease in the expression of miR-449a, a conserved miRNA highly enriched in ciliated airway epithelia and implicated in motile ciliogenesis, was observed in CS-exposed cultures. Similar alterations in miR-449a have been reported in smokers with COPD. Network analysis further indicates that downregulation of miR-449a by CS may derepress cell-cycle proteins, which, in turn, interferes with ciliogenesis. Investigating the effects of CS on transcriptome profile in human ALI cultures may provide not only mechanistic insights, but potential early biomarkers for CS exposure and harm.
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