期刊
AQUACULTURE
卷 532, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2020.736072
关键词
Atlantic cod larvae; Probiotic; Carnobacterium divergens; Vibrio anguillarum; 16S rRNA sequencing; Microbiota
资金
- Research Council of Norway [227356]
The study demonstrated that feeding Atlantic cod larvae with two Carnobacterium divergens strains significantly improved growth, survival, and disease resistance. The enrichment of carnobacteria in the feed did not significantly impact the overall microbiota diversity or composition. Despite this, the probiotic treatment positively affected the performance and welfare of cod larvae.
Aquaculture represents the fastest growing food sector and the second largest export commodity in Norway. Disease in aquaculture causes mortality and huge losses in farmed fish. Probiotic treatment of farmed fish, i.e. feeding of fish with live, beneficial microorganisms, may represent one part of a sustainable solution to these problems. Some carnobacteria of fish origin have shown promising results as probiotics for fish. In this study, two Carnobacterium divergens strains were fed to Atlantic cod (Gadus morhua) larvae through intermittent feeding with Artemia franciscana containing the bacterial strains. Cod larvae fed with the carnobacteria showed a significantly higher growth and survival during the larval rearing stage compared to the larvae fed A. franciscana with no carnobacteria (control treatment). Following the probiotic feeding period, the cod larvae were challenged with a pathogenic bacterium, Vibrio anguillarum. Cod larvae from probiotic treatment showed significantly better disease resistance compared to the control. Microbiota analysis by 16S rRNA amplicon sequencing verified that enrichment of carnobacteria in the A. franciscana feed. Analysis of the whole larvae detected only minor relative levels of carnobacteria in the probiotic treated larvae, which was non-significant compared to the control larvae (p = 0.062). The probiotic treatment had no significant impact on the overall microbiota diversity or composition in the larvae during the probiotic feeding period or after the disease challenge. Despite this, significantly improved growth and survival during larval rearing and post-pathogen challenge suggest probiotic effects of C. divergens mixture on cod larval fish performance and welfare. Studies examining the mode of action should be carried out to get more insight to lead to the commercial application of C. divergens in Atlantic cod larviculture.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据