期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 105, 期 4, 页码 1745-1758出版社
SPRINGER
DOI: 10.1007/s00253-021-11130-3
关键词
Yarrowia lipolytica; Adaptive laboratory evolution; Transcriptome analysis; Ferulic acid tolerance; Vanillic acid tolerance; Reverse metabolic engineering
资金
- Natural Science Foundation of Jiangsu Province [BK20170037, BK20170829]
- National Key R&D Program of China [2016YFE0105400]
- National Natural Science Foundation of China [21606132, 21706133]
Yarrowia lipolytica strain was evolved through adaptive laboratory evolution to increase tolerance to ferulic acid, leading to a new strain with improved viability under high concentrations of ferulic acid, showing promise for application in lignocellulose conversion.
Yarrowia lipolytica strain is a promising cell factory for the conversion of lignocellulose to biofuels and bioproducts. Despite the inherent robustness of this strain, further improvements to lignocellulose-derived inhibitors toxicity tolerance of Y. lipolytica are also required to achieve industrial application. Here, adaptive laboratory evolution was employed with increasing concentrations of ferulic acid. The adaptive laboratory evolution experiments led to evolve Y. lipolytica strain yl-XYL + *FA*4 with increased tolerance to ferulic acid as compared to the parental strain. Specifically, the evolved strain could tolerate 1.5 g/L ferulic acid, whereas 0.5 g/L ferulic acid could cause about 90% lethality of the parental strain. Transcriptome analysis of the evolved strain revealed several targets underlying toxicity tolerance enhancements. YALI0_E25201g, YALI0_F05984g, YALI0_B18854g, and YALI0_F16731g were among the highest upregulated genes, and the beneficial contributions of these genes were verified via reverse engineering. Recombinant strains with overexpressing each of these four genes obtained enhanced tolerance to ferulic acid as compared to the control strain. Fortunately, recombinant strains with overexpression of YALI0_E25201g, YALI0_B18854g, and YALI0_F16731g individually also obtained enhanced tolerance to vanillic acid. Overall, this work demonstrated a whole strain improvement cycle by non-rational metabolic engineering and presented new targets to modify Y. lipolytica for microbial lignocellulose valorization.
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