4.7 Article

Artemisinin-Based Drugs Target the Plasmodium falciparum Heme Detoxification Pathway

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出版社

AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.02137-20

关键词

dihydroartemisinin; artesunate; artemether; hemozoin; synergy

资金

  1. NIH [R01 56312, R21 146506]
  2. Georgetown Chemistry Department

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Artemisinin-based antimalarial drugs are believed to exert lethal effects on malarial parasites by alkylating a variety of intracellular molecular targets, with recent studies showing that these drugs can inhibit the parasite's detoxification of free heme. The experiments indicate that artemisinins have a strong inhibitory effect on hemozoin crystallization and are more potent inhibitors compared to quinoline-based antimalarial drugs.
Artemisinin (ART)-based antimalarial drugs are believed to exert lethal effects on malarial parasites by alkylating a variety of intracellular molecular targets. Recent work with live parasites has shown that one of the alkylated targets is free heme within the parasite digestive vacuole, which is liberated upon hemoglobin catabolism by the intraerythrocytic parasite, and that reduced levels of heme alkylation occur in artemisinin-resistant parasites. One implication of heme alkylation is that these drugs may inhibit parasite detoxification of free heme via inhibition of hemeto-hemozoin crystallization; however, previous reports that have investigated this hypothesis present conflicting data. By controlling reducing conditions and, hence, the availability of ferrous versus ferric forms of free heme, we modify a previously reported hemozoin inhibition assay to quantify the ability of ART-based drugs to target the heme detoxification pathway under reduced versus oxidizing conditions. Contrary to some previous reports, we find that artemisinins are potent inhibitors of hemozoin crystallization, with effective half-maximal concentrations approximately an order of magnitude lower than those for most quinoline-based antimalarial drugs. We also examine hemozoin and in vitro parasite growth inhibition for drug pairs IV found in the most commonly used ART-based combination therapies (ACTs). All ACTs examined inhibit hemozoin crystallization in an additive fashion, and all but one inhibit parasite growth in an additive fashion.

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