Enhanced Ultrasound Contrast of Renal-Clearable Luminescent Gold Nanoparticles

Renal-clearable nanoparticles are typically fast eliminated through the free glomerular filtration, which show weak interaction with the renal compartments and negligible ultrasound signals, raising challenges in direct imaging of kidney diseases. Here, we report the ultrasmall renal-clearable luminescent gold nanoparticles (AuNPs) with both pH-induced charge reversal and aggregation properties, and discover that enhanced ultrasound contrast could be facilely acquired through the increased tubular reabsorption and in situ aggregation of AuNPs in renal tubule cells in injured kidneys. The tuning elimination pathway of the renal-clearable luminescent AuNPs is further demonstrated to provide a synergistical fluorescence and ultrasound imaging strategy for diagnosing early kidney injury with precise anatomical information.

Automated summary

This study introduces ultrasmall renal-clearable luminescent gold nanoparticles with pH-induced charge reversal and aggregation properties, which enhance ultrasound contrast in injured kidneys through increased tubular reabsorption and in situ aggregation. The tuning elimination pathway of these nanoparticles provides a synergistical fluorescence and ultrasound imaging strategy for diagnosing early kidney injury with precise anatomical information.