Spatially Selective Imaging of Cell-Matrix and Cell-Cell Junctions by Electrochemiluminescence

Cell junctions are protein structures located at specific cell membrane domains that determine key processes in multicellular development. Here we report spatially selective imaging of cell junctions by electrochemiluminescence (ECL) microscopy. By regulating the concentrations of luminophore and/or co-reactant, the thickness of ECL layer can be controlled to match with the spatial location of different cell junctions. At a low concentration of luminophore, ECL generation is confined to the electrode surface, thus revealing only cell-matrix adhesions at the bottom of cells. While at a high concentration of luminophore, the ECL layer can be remarkably extended by decreasing the co-reactant concentration, thus allowing the sequential imaging of cell-matrix and cell-cell junctions at the bottom and near the apical surface of cells, respectively. This strategy not only provides new insights into the ECL mechanisms but also promises wide applications of ECL microscopy in bioimaging.

Automated summary

A novel method for spatially selective imaging of cell junctions by regulating the thickness of the electrochemiluminescence (ECL) layer through control of luminophore and co-reactant concentrations has been reported. Low luminophore concentration reveals cell-matrix adhesions, while high concentration allows sequential imaging of cell-matrix and cell-cell junctions. This strategy not only provides new insights into ECL mechanisms but also expands the potential applications of ECL microscopy in bioimaging.