期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 21, 页码 11784-11788出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202102001
关键词
cell death; imaging; lipid; mass spectrometry; oxidation
资金
- National Institutes of Health [R01NS076511, R01NS061817, U01AI156924, P01HL114453, R01CA165065, R01AI145406, U01AI156923, R01CA243142, R01GM134715, R01GM113908, R01HL151078, R01NS117000, U54GM103529-08]
- Natural Science Foundation of China [81873209, 81703696]
- 111 Project of Chinese MoE [B13038]
Using gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) imaging, peroxidized phosphatidylethanolamine (PEox) could be mapped with high spatial resolution in ferroptotic cardiomyocytes and neurons after traumatic brain injury, revealing their accumulation at very low physiological levels in subcellular compartments.
Peroxidized phosphatidylethanolamine (PEox) species have been identified by liquid chromatography mass spectrometry (LC-MS) as predictive biomarkers of ferroptosis, a new program of regulated cell death. However, the presence and subcellular distribution of PEox in specific cell types and tissues have not been directly detected by imaging protocols. By applying gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) imaging with a 70 keV (H2O)(n)(+) (n>28 000) cluster ion beam, we were able to map PEox with 1.2 mu m spatial resolution at the single cell/subcellular level in ferroptotic H9c2 cardiomyocytes and cortical/hippocampal neurons after traumatic brain injury. Application of this protocol affords visualization of physiologically relevant levels of very low abundance (20 pmol mu mol(-1) lipid) peroxidized lipids in subcellular compartments and their accumulation in disease conditions.
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