期刊
ANALYTICAL SCIENCES
卷 37, 期 3, 页码 491-497出版社
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.20SCP10
关键词
Artificial basement membranes; thickness; endothelial cell function
资金
- Bilateral Joint Research Projects of the Japan Society for the Promotion of Science [20199946]
- AMED-MPS [19be0304207h003]
- [20H00665]
The roughness of A-BMs increases gradually with the increase of nanofilm thickness, leading to the inhibition of EC adhesion, spreading, and proliferation. Increasing seeding cell number and expanding culture time can compensate for the interendothelial junctions and barrier effect of confluent EC monolayers on thicker A-BM surfaces.
Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues.
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