期刊
ANALYTICAL LETTERS
卷 54, 期 17, 页码 2801-2811出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2021.1891547
关键词
Cyanobacteria; free fatty acids; stir bar sorptive extraction (SBSE); ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS; MS)
资金
- National Natural Science Foundation of China [21305149]
A rapid and sensitive method was developed for the determination of free fatty acids in cyanobacteria extracellular media without derivatization by SBSE-UHPLC-MS/MS. The method successfully extracted and quantified 11 free fatty acids, providing important reference for further studies.
A rapid and sensitive method was developed determine free fatty acids in cyanobacteria extracellular medium without derivatization by stir bar sorptive extraction (SBSE) coupled to ultra-high-performance liquid chromatography - tandem mass spectrometry (UHPLC-MS/MS). The stir bars (20 mm in length and 1 mm in film thickness) coated with polydimethylsiloxane was used to directly extract 11 free fatty acids (C-14-C-24) in cyanobacteria extracellular media. The performance was optimized, including the column, temperature, extraction time, organic modifier, and desorption solvent. Limits of detection and quantification, precision, and linear dynamic ranges for the free fatty acids were evaluated. The method was applied to determine free fatty acids in cyanobacteria extracellular media at the exponential and plateau phases. Eicosanoic acid (C20:0), linoleic acid (C18:2), oleic acid (C18:1), stearic acid (C18:0), palmitic acid (C16:0) and myristic acid (C14:0) were quantified in the samples.
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