期刊
ANALYTICAL CHEMISTRY
卷 93, 期 8, 页码 3976-3986出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c04952
关键词
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资金
- Spinnaker Health Research Foundation, WA
- McCusker Foundation, WA
- Western Australian State Government
- MRFF
- UK MRC
- Department of Jobs, Tourism, Science and Innovation, Government of Western Australian Premier's Fellowship
- ARC
Nuclear magnetic resonance spectroscopy-based plasma phenotyping using DIRE technique successfully revealed diagnostic molecular signatures of SARS-CoV-2 infection in plasma. Comparison of plasma samples from different groups showed differences in concentrations of specific metabolites in SARS-CoV-2 positive patients, suggesting potential molecular markers for COVID-19 diagnosis.
We have applied nuclear magnetic resonance spectroscopy based plasma phenotyping to reveal diagnostic molecular signatures of SARS-CoV-2 infection via combined diffusional and relaxation editing (DIRE). We compared plasma from healthy age-matched controls (n = 26) with SARS-CoV-2 negative non-hospitalized respiratory patients and hospitalized respiratory patients (n = 23 and 11 respectively) with SARS-CoV-2 rRT-PCR positive respiratory patients (n = 17, with longitudinal sampling time-points). DIRE data were modelled using principal component analysis and orthogonal projections to latent structures discriminant analysis (O-PLS-DA), with statistical cross-validation indices indicating excellent model generalization for the classification of SARS-CoV-2 positivity for all comparator groups (area under the receiver operator characteristic curve = 1). DIRE spectra show biomarker signal combinations conferred by differential concentrations of metabolites with selected molecular mobility properties. These comprise the following: (a) composite N-acetyl signals from alpha-1-acid glycoprotein and other glycoproteins (designated GlycA and GlycB) that were elevated in SARS-CoV-2 positive patients [p = 2.52 x 10(-10) (GlycA) and 1.25 x 10(-9) (GlycB) vs controls], (b) two diagnostic supramolecular phospholipid composite signals that were identified (SPC-A and SPC-B) from the -N+-(CH3)(3) choline headgroups of lysophosphatidylcholines carried on plasma glycoproteins and from phospholipids in high-density lipoprotein subfractions (SPC-A) together with a phospholipid component of low-density lipoprotein (SPC-B). The integrals of the summed SPC signals (SPCtotal) were reduced in SARS-CoV-2 positive patients relative to both controls (p = 1.40 x 10(-7)) and SARS-CoV-2 negative patients (p = 4.52 x 10(-8)) but were not significantly different between controls and SARS-CoV-2 negative patients. The identity of the SPC signal components was determined using one and two dimensional diffusional, relaxation, and statistical spectroscopic experiments. The SPCtotal/GlycA ratios were also significantly different for control versus SARS-CoV-2 positive patients (p = 1.23 x 10(-10)) and for SARS-CoV-2 negatives versus positives (p = 1.60 x 10(-9)). Thus, plasma SPCtotal and SPCtotal/GlycA are proposed as sensitive molecular markers for SARS-CoV-2 positivity that could effectively augment current COVID-19 diagnostics and may have value in functional assessment of the disease recovery process in patients with long-term symptoms.
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