期刊
ANALYTICAL CHEMISTRY
卷 93, 期 8, 页码 3778-3785出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c04200
关键词
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资金
- National Research Foundation (NRF) - Ministry of Science and ICT of South Korea [NRF-2018R1C1B6007391]
- Korea Institute of Materials Science (KIMS) [PNK 7440]
Metabolomics has shown great potential for early cancer diagnosis, and a rapid urine analysis system based on SERS has been developed for the diagnosis of pancreatic cancer and prostate cancer. The results indicate that the system has great potential for noninvasive cancer diagnosis and screening.
Metabolomics shows tremendous potential for the early diagnosis and screening of cancer. For clinical application as an effective diagnostic tool, however, improved analytical methods for complex biological fluids are required. Here, we developed a reliable rapid urine analysis system based on surface-enhanced Raman spectroscopy (SERS) using 3D-stacked silver nanowires (AgNWs) on a glass fiber filter (GFF) sensor and applied it to the diagnosis of pancreatic cancer and prostate cancer. Urine samples were pretreated with centrifugation to remove large debris and with calcium ion addition to improve the binding of metabolites to AgNWs. The label-free urine-SERS detection using the AgNW-GFF SERS sensor showed different spectral patterns and distinguishable specific peaks in three groups: normal control (n = 30), pancreatic cancer (n = 22), and prostate cancer (n = 22). Multivariate analyses of SERS spectra using unsupervised principal component analysis and supervised orthogonal partial least-squares discriminant analysis showed excellent discrimination between the pancreatic cancer group and the prostate cancer group as well as between the normal control group and the combined cancer groups. The results demonstrate the great potential of the urine-SERS analysis system using the AgNW-GFF SERS sensor for the noninvasive diagnosis and screening of cancers.
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