4.8 Article

Bioinspired Artificial Clickase for the Catalytic Click Immunoassay of Foodborne Pathogens

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Summary: A novel laccase mimics, LM nanozymes, with superior catalytic activity were successfully prepared and characterized, showing advantages over natural laccase in terms of stability, cost, activity, and substrate affinity. LM nanozymes were utilized in a nanozyme-based ELISA for alpha-lactalbumin detection, achieving high sensitivity and specificity with a portable detection method integrated with a smartphone. This work not only enriches the diversity of nanozymes but also expands their promising applications in biosensing.

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