期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 413, 期 18, 页码 4619-4623出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03193-y
关键词
Fluorescence polarization immunoassay; Avian influenza; Virus detection; Rapid diagnosis
资金
- JST-SENTAN program [JPMJSN16A2]
- Hokkaido University Research and Education Center for Robust Agriculture, Forestry, and Fisheries Industry
- Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering
- Cooperative Research Program of NJRC Mater. Dev. [20J11226]
- JSPS [20J11226]
- Grants-in-Aid for Scientific Research [20J11226] Funding Source: KAKEN
Researchers developed a non-competitive fluorescence polarization immunoassay (NC-FPIA) for detecting H5 subtype avian influenza virus, achieving successful detection within 15 minutes and enabling rapid on-site diagnosis of various viruses.
Nowadays, the diagnosis of viral infections is receiving broad attention. We have developed a non-competitive fluorescence polarization immunoassay (NC-FPIA), which is a separation-free immunoassay, for a virus detection. H5 subtype avian influenza virus (H5-AIV) was used as a model virus for the proof of concept. The fluorescein-labeled Fab fragment that binds to H5 hemagglutinin was used for NC-FPIA. The purified H5-AIV which has H5 hemagglutinin was mixed with the fluorescein-labeled Fab fragment. After that, the degree of fluorescence polarization was measured with a portable FPIA analyzer. H5-AIV was successfully detected with an incubation time of 15 min. In addition, the portable FPIA analyzer enables performance of on-site NC-FPIA with a sample volume of 20 mu L or less. This is the first research of detecting a virus particle by FPIA. This NC-FPIA can be applied to rapid on-site diagnosis of various viruses.
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