No Evidence of rVSV-Ebola Virus Vaccine Replication or Dissemination in the Sand Fly Phlebotomus papatasi

Following vaccination with the live attenuated, recombinant vesicular stomatitis virus Indiana serotype Ebola virus (rVSV-EBOV) vaccine, persons may exhibit a transient vaccine-associated viremia. To investigate the potential for Old World sand flies to transmit this vaccine following feeding on a viremic person, we fed laboratory-reared Phlebotomus papatasi an artificial blood meal containing 7.2 log10 plaque-forming units of rVSV-EBOV. Replication or dissemination was not detected in the body or legs of any P. papatasi collected at seven (n = 75) or 15 (n = 75) days post-feed. These results indicate a low potential for rVSV-EBOV to replicate and disseminate in P. papatasi, a species whose geographic distribution ranges from Morocco to southwest Asia and as far north as southern Europe.

Automated summary

Following feeding on an artificial blood meal containing rVSV-EBOV, laboratory-reared Phlebotomus papatasi did not show replication or dissemination of the vaccine virus in their bodies or legs at seven or 15 days post-feed. This indicates a low potential for rVSV-EBOV to replicate and disseminate in P. papatasi, a species with geographic distribution ranging from Morocco to southwest Asia and southern Europe.