4.4 Article

Evidence for anti-inflammatory effects of firocoxib administered to mares with experimentally induced placentitis

期刊

出版社

WILEY
DOI: 10.1111/aji.13396

关键词

anti‐ inflammatory; cytokine; equine; firocoxib; placentitis; pregnancy; prostaglandin

资金

  1. Kentucky Thoroughbred Owners and Breeders Association
  2. Grayson-Jockey Club Research Foundation
  3. Merial Animal Health
  4. University of Florida, College of Veterinary Medicine

向作者/读者索取更多资源

The study suggests that administration of firocoxib has a suppressive effect on cytokine and prostaglandin production in mares with placentitis.
Problem Minimal evidence exists supporting therapeutic selections for equine placentitis. The goal of this study was to characterize the anti-inflammatory effects of firocoxib when administered to mares with placentitis. Methods Mares (gestation D270-300) were assigned to: INFECT (n = 6; placentitis, no treatment), FIRO (n = 6; placentitis, firocoxib, 0.1 mg/kg, PO, daily), and NORM (n = 6; no infection/treatment). Allantoic fluid (8 hours, 24 hours, birth) and amniotic fluid (birth) were collected from mares after infection. Concentrations of IL-1 beta, IL-6, TNF-alpha, IL-10, PGF(2 alpha), and PGE(2) in fluids were measured by ELISA. mRNA expression of IL-1 beta, IL-6, TNF-alpha, IL-8, IL-10, matrix metalloproteinases (MMPs) -1, 3, and 9 in fetal membranes/fetuses was quantified using real-time PCR. Results Allantoic TNF-alpha concentrations were lowest in FIRO at 8 hours and 24 hours post-infection; IL-6 concentrations were lower in FIRO than NORM at 8 hours, lower in FIRO than INFECT at 24 hours post-inoculation, and lower in NORM than FIRO or INFECT at birth. Marginal mean allantoic IL-beta and IL-10 concentrations were lower in FIRO and NORM than INFECT. Amniotic fluid cytokines were lowest in NORM with all measurements in that group being below the limit of detection. Allantoic PGF(2 alpha) concentrations were lower in FIRO and INFECT than NORM at 8 hours post-inoculation, and lower in FIRO than INFECT or NORM at 24 hours post-inoculation. Allantoic PGE(2) concentrations were lower in FIRO than INFECT. Amniotic PGF(2 alpha) and PGE(2) concentrations were lower in NORM than INFECT. In fetal membranes, group differences with respect to IL-1 beta, IL-6, IL-8, and MMP1 were dependent on tissue type. Conclusions Data suggest a suppressive effect of firocoxib administration on cytokine and prostaglandin production in mares with placentitis.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据