期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 320, 期 4, 页码 H1670-H1686出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00941.2020
关键词
contractility; engineered cardiac tissue; human iPS cells; maturation; t-tubules
资金
- University of Wisconsin Translational Research Initiatives in Pathology laboratory (TRIP)
- UW Department of Pathology and Laboratory Medicine, UWCCC [P30 CA014520]
- Office of The Director-NIH [S10OD023526]
- Wisconsin Partnership Program
This study provides a detailed assessment of engineered human cardiac tissue constructs (hiPSC-ECTs) formed by coculturing hiPSC-CMs and cardiac fibroblasts in a 3D fibrin matrix. The hiPSC-ECTs show improved physiological responsiveness, develop a t-tubular system, and exhibit calcium-handling and contractile kinetics similar to human myocardium. The study emphasizes the suitability of 3D-engineered human cardiac tissues as a model for studying cardiac function and provides data to aid in selecting the most appropriate configuration based on the research question.
Cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CM) may provide an important bridge between animal models and the intact human myocardium. Fulfilling this potential is hampered by their relative immaturity, leading to poor physiological responsiveness. hiPSC-CMs grown in traditional two-dimensional (2D) culture lack a t-tubular system, have only rudimentary intracellular calcium-handling systems, express predominantly embryonic sarcomeric protein isoforms, and preferentially use glucose as an energy substrate. Culturing hiPSC-CM in a variety of three-dimensional (3D) environments and the addition of nutritional, pharmacological, and electromechanical stimuli have proven, to various degrees, to be beneficial for maturation. We present a detailed assessment of a novel model in which hiPSC-CMs and hiPSC-derived cardiac fibroblasts are cocultured in a 3D fibrin matrix to form engineered cardiac tissue constructs (hiPSC-ECTs). The hiPSC-ECTs are responsive to physiological stimuli, including stretch, frequency, and b-adrenergic stimulation, develop a t-tubular system, and demonstrate calcium-handling and contractile kinetics that compare favorably with ventricular human myocardium. Furthermore, transcript levels of various genes involved in calcium-handling and contraction are increased. These markers of maturation become more robust over a relatively short period of time in culture (6 wk vs. 2wk in hiPSC-ECTs). A comparison of the hiPSC-ECT molecular and performance variables with those of human cardiac tissue and other available engineered tissue platforms is provided to aid selection of the most appropriate platform for the research question at hand. Important and noteworthy aspects of this human cardiac model system are its reliance on off-the-shelf equipment, ability to provide detailed physiological performance data, and the ability to achieve a relatively mature cardiac physiology without additional nutritional, pharmacological, and electromechanical stimuli that may elicit unintended effects on function. NEW & NOTEWORTHY This study seeks to provide an in-depth assessment of contractile performance of human iPSC-derived cardiomyocytes cultured together with fibroblasts in a 3-dimensional-engineered tissue and compares performance both over time as cells mature, and with corresponding measures found in the literature using alternative 3D culture configurations. The suitability of 3D-engineered human cardiac tissues to model cardiac function is emphasized, and data provided to assist in the selection of the most appropriate configuration based on the target application.
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